Nitrogen occurrence forms and bacterial community in sediment influenced by Bellamya purificata bioturbation

Endogenous water pollution and excessive nitrogen in ponds have always been prominent problems threatening aquaculture. To solve this problem, a 70-day indoor simulation experiment was conducted. The snail Bellamya purificata stocked at four different densities: 0, 15, 30, 60 individuals/tank, represented as CON (control), LD (low density), MD (medium HD (high respectively, to investigate the effects of B. on occurrence forms bacterial communities sediment. At end experiment, nitrate concentration significantly higher, while total content lower group than other three groups. Ammonia monooxygenase activity groups, hydroxylamine oxidase higher groups showed highest lowest values reductase activity, respectively. decreased with increasing density. Through 16S ribosomal RNA (rRNA) high-throughput sequencing, affected by were found. Alpha diversity results that, Shannon index observed Chao1 values, Phyla Nitrospinae family Nitrosomonadaceae enriched Redundancy analysis (RDA) indicated significant correlation between differential taxa TN content. Predicted functional based FAPROTAX (Functional annotation prokaryotic taxa) database that aerobic ammonia oxidation nitrite group. Overall, altered community composition, increased activities, enhanced nitrification process, promoted transformation nitrate. Moreover, 30 individuals/tank appeared promotion effect Hence, co-culturing may be feasible effective ecological restoration method alleviate excess reduce eutrophication.